polyclonal rabbit anti-human cxcr2  (Santa Cruz Biotechnology)

Journal: Cell Reports Methods

Article Title: Discovery of antibodies targeting multipass transmembrane proteins using a suspension cell-based evolutionary approach

doi: 10.1016/j.crmeth.2023.100429

Figure Lengend Snippet: Hybrid adherent/suspension cell-based selection strategy leads to discovery of nanobodies against the GPCR CXCR2

Article Snippet: A final competition assay was carried out similarly, titrating either the 10H2 antibody, the CX2_1 nanobody-Fc fusion protein, the IL-8 chemokine, or an irrelevant negative control antibody (tocilizumab) in the presence and absence of a rabbit anti-human CXCR2 polyclonal antibody (Fabgennix, product number CXCR2-201AP) at a 1:50 dilution (133 nM).

Techniques: Selection

Journal: Cell Reports Methods

Article Title: Discovery of antibodies targeting multipass transmembrane proteins using a suspension cell-based evolutionary approach

doi: 10.1016/j.crmeth.2023.100429

Figure Lengend Snippet: Key resources table

Article Snippet: A final competition assay was carried out similarly, titrating either the 10H2 antibody, the CX2_1 nanobody-Fc fusion protein, the IL-8 chemokine, or an irrelevant negative control antibody (tocilizumab) in the presence and absence of a rabbit anti-human CXCR2 polyclonal antibody (Fabgennix, product number CXCR2-201AP) at a 1:50 dilution (133 nM).

Techniques: Recombinant, Expressing, Modification, Infection, Transfection, Affinity Chromatography, Transduction, Plasmid Preparation, Sequencing, Software

Journal: Cell Reports Methods

Article Title: Discovery of antibodies targeting multipass transmembrane proteins using a suspension cell-based evolutionary approach

doi: 10.1016/j.crmeth.2023.100429

Figure Lengend Snippet: Hybrid adherent/suspension cell-based selection strategy leads to discovery of nanobodies against the GPCR CXCR2

Article Snippet: A final competition assay was carried out similarly, titrating either the 10H2 antibody, the CX2_1 nanobody-Fc fusion protein, the IL-8 chemokine, or an irrelevant negative control antibody (tocilizumab) in the presence and absence of a rabbit anti-human CXCR2 polyclonal antibody (Fabgennix, product number CXCR2-201AP) at a 1:50 dilution (133 nM).

Techniques: Suspension, Selection

Journal: Cell Reports Methods

Article Title: Discovery of antibodies targeting multipass transmembrane proteins using a suspension cell-based evolutionary approach

doi: 10.1016/j.crmeth.2023.100429

Figure Lengend Snippet: Key resources table

Article Snippet: A final competition assay was carried out similarly, titrating either the 10H2 antibody, the CX2_1 nanobody-Fc fusion protein, the IL-8 chemokine, or an irrelevant negative control antibody (tocilizumab) in the presence and absence of a rabbit anti-human CXCR2 polyclonal antibody (Fabgennix, product number CXCR2-201AP) at a 1:50 dilution (133 nM).

Techniques: Virus, Recombinant, Expressing, Modification, Infection, Transfection, Affinity Chromatography, Transduction, Plasmid Preparation, Sequencing, Software

Journal: Cell Reports Methods

Article Title: Discovery of antibodies targeting multipass transmembrane proteins using a suspension cell-based evolutionary approach

doi: 10.1016/j.crmeth.2023.100429

Figure Lengend Snippet: Hybrid adherent/suspension cell-based selection strategy leads to discovery of nanobodies against the GPCR CXCR2

Article Snippet: Rabbit anti-human CXCR2 polyclonal , Fabgennix , Cat#CXCR2-201AP.

Techniques: Suspension, Selection

Journal: Cell Reports Methods

Article Title: Discovery of antibodies targeting multipass transmembrane proteins using a suspension cell-based evolutionary approach

doi: 10.1016/j.crmeth.2023.100429

Figure Lengend Snippet: Key resources table

Article Snippet: Rabbit anti-human CXCR2 polyclonal , Fabgennix , Cat#CXCR2-201AP.

Techniques: Virus, Recombinant, Expressing, Modification, Infection, Transfection, Affinity Chromatography, Transduction, Plasmid Preparation, Sequencing, Software

Journal:

Article Title: Downregulation of CXCR1 and CXCR2 Expression on Human Neutrophils by Helicobacter pylori : a New Pathomechanism in H. pylori Infection?

doi: 10.1128/IAI.72.12.6773-6779.2004

Figure Lengend Snippet: H. pylori downregulates the CXCR1 and CXCR2 receptors on purified human neutrophils. Incubation of neutrophils with a cag+ H. pylori strain as well as with the corresponding cag deletion mutant resulted in complete downregulation of both chemokine receptors after only 0.5 h, as determined by FACS analysis. Neutrophils incubated with medium alone (labeled “medium control”) did not show any receptor downregulation. The x axis indicates fluorescence intensity measured on log10 scale, and the y axis indicates event counts per channel on a linear scale.

Article Snippet: The blotted membranes were blocked with phosphate-buffered saline containing 0.1% (vol/vol) Tween 20 and 3% (wt/vol) low-fat milk powder, followed by a 1-h incubation with the primary antibodies polyclonal rabbit anti-human CXCR1 and CXCR2 (Santa Cruz, Heidelberg, Germany) at a dilution of 1:200.

Techniques: Purification, Incubation, Mutagenesis, Labeling, Fluorescence

Journal:

Article Title: Downregulation of CXCR1 and CXCR2 Expression on Human Neutrophils by Helicobacter pylori : a New Pathomechanism in H. pylori Infection?

doi: 10.1128/IAI.72.12.6773-6779.2004

Figure Lengend Snippet: Purified LPS from H. pylori does not downregulate the CXCR1 and CXCR2 receptors on human neutrophils. Incubation of neutrophils with LPS from H. pylori did not downregulate the CXCR1 and CXCR2 receptors compared with the medium control, as determined by FACS analysis.

Article Snippet: The blotted membranes were blocked with phosphate-buffered saline containing 0.1% (vol/vol) Tween 20 and 3% (wt/vol) low-fat milk powder, followed by a 1-h incubation with the primary antibodies polyclonal rabbit anti-human CXCR1 and CXCR2 (Santa Cruz, Heidelberg, Germany) at a dilution of 1:200.

Techniques: Purification, Incubation

Journal:

Article Title: Downregulation of CXCR1 and CXCR2 Expression on Human Neutrophils by Helicobacter pylori : a New Pathomechanism in H. pylori Infection?

doi: 10.1128/IAI.72.12.6773-6779.2004

Figure Lengend Snippet: CXCR1 and CXCR2 receptor downregulation on neutrophils cannot be attributed to increased IL-8 and TNF-α concentrations. Human neutrophils did not express IL-8 (a) and TNF-α (b) 0.5 h after incubation with H. pylori, as determined by ELISA. Three hours after incubation with H. pylori, IL-8 was strongly increased and TNF-α was moderately increased in the neutrophil cell culture. The IL-8 level was higher after incubation with the cag+ than with the cag deletion H. pylori strain. IL-8 measurements were performed in triplicate, and TNF-α measurements were performed in duplicate. Mean values and standard deviations are shown.

Article Snippet: The blotted membranes were blocked with phosphate-buffered saline containing 0.1% (vol/vol) Tween 20 and 3% (wt/vol) low-fat milk powder, followed by a 1-h incubation with the primary antibodies polyclonal rabbit anti-human CXCR1 and CXCR2 (Santa Cruz, Heidelberg, Germany) at a dilution of 1:200.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Cell Culture

Journal:

Article Title: Downregulation of CXCR1 and CXCR2 Expression on Human Neutrophils by Helicobacter pylori : a New Pathomechanism in H. pylori Infection?

doi: 10.1128/IAI.72.12.6773-6779.2004

Figure Lengend Snippet: H. pylori downregulates the CXCR1 and CXCR2 receptors on neutrophils, as shown by confocal microscopy. Purified human neutrophils showed strong CXCR1 and CXCR2 expression at the cell membrane and in the cytoplasm after 0.5 h of incubation in RPMI medium. Incubation with a cag+ and a cag deletion H. pylori strain for 0.5 h resulted in a strong reduction of the fluorescence signals, with complete loss of the signals at the cell membrane and only some CXCR1 and CXCR2 signal in the cytoplasm. Fluorescence microscopy is shown in the lower part of the figure, and transmission light microscopy of the same cells is shown in the upper part of the figure.

Article Snippet: The blotted membranes were blocked with phosphate-buffered saline containing 0.1% (vol/vol) Tween 20 and 3% (wt/vol) low-fat milk powder, followed by a 1-h incubation with the primary antibodies polyclonal rabbit anti-human CXCR1 and CXCR2 (Santa Cruz, Heidelberg, Germany) at a dilution of 1:200.

Techniques: Confocal Microscopy, Purification, Expressing, Incubation, Fluorescence, Microscopy, Transmission Assay, Light Microscopy

Journal:

Article Title: Downregulation of CXCR1 and CXCR2 Expression on Human Neutrophils by Helicobacter pylori : a New Pathomechanism in H. pylori Infection?

doi: 10.1128/IAI.72.12.6773-6779.2004

Figure Lengend Snippet: cag+ H. pylori induced more severe downregulation of CXCR1 and CXCR2 mRNAs in purified human neutrophils than cag deletion H. pylori. Neutrophils incubated in RPMI for 0.5 and 3 h strongly expressed CXCR1 and CXCR2 mRNAs (lanes 1 and 4). H. pylori downregulated CXCR1 and CXCR2 mRNAs in neutrophils 3 h after incubation (lanes 5 and 6). CXCR1 and CXCR2 mRNAs were not downregulated 0.5 h after coincubation with H. pylori (lanes 2 and 3). The H. pylori cag+ strain (lane 5) downregulated CXCR1 and CXCR2 mRNAs significantly more strongly than the cag deletion mutant (lane 6).

Article Snippet: The blotted membranes were blocked with phosphate-buffered saline containing 0.1% (vol/vol) Tween 20 and 3% (wt/vol) low-fat milk powder, followed by a 1-h incubation with the primary antibodies polyclonal rabbit anti-human CXCR1 and CXCR2 (Santa Cruz, Heidelberg, Germany) at a dilution of 1:200.

Techniques: Purification, Incubation, Mutagenesis

Journal:

Article Title: Downregulation of CXCR1 and CXCR2 Expression on Human Neutrophils by Helicobacter pylori : a New Pathomechanism in H. pylori Infection?

doi: 10.1128/IAI.72.12.6773-6779.2004

Figure Lengend Snippet: Downmodulation of the CXCR1 receptor on neutrophils may also occur during H. pylori infection in the human stomach. Neutrophils in the lamina propria expressed the CXCR1 receptor, whereas neutrophils infiltrating gastric epithelium or localized in the foveolar lumen (arrows), where they are in closer contact with H. pylori, lacked CXCR1 expression.

Article Snippet: The blotted membranes were blocked with phosphate-buffered saline containing 0.1% (vol/vol) Tween 20 and 3% (wt/vol) low-fat milk powder, followed by a 1-h incubation with the primary antibodies polyclonal rabbit anti-human CXCR1 and CXCR2 (Santa Cruz, Heidelberg, Germany) at a dilution of 1:200.

Techniques: Infection, Expressing